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Ideal to support periods of increased workload or training

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RNA Extraction and Real-Time Polymerase Chain Reaction Assay Total RNA was extracted from frozen jejunum samples with TRIzol reagent (Sigma-Aldrich), and was used to synthesize cDNA with Prime-Script RT reagent kit (Takara, Dalian, China) according to the manufacturers instructions

Primary infection was considered less likely given the absence of systemic symptoms and persistence despite systemic antibiotics, although secondary impetiginization could not be excluded