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Due to their enrichment in polyunsaturated phospholipids, peroxisomal membranes are highly vulnerable to 4-HNE-induced oxidative damage, which inactivates membrane-associated proteins such as PMP70 and PEX5 and disrupts membrane integrity, giving rise to leaky peroxisomes. The uncontrolled release of ROS and matrix proteins into the cytosol intensifies oxidative stress and, through damaged mitochondriaperoxisome contact sites, impairs fatty acid trafficking and metabolic coordination, thereby aggravating lipotoxic injury in DCM

The Krebs cycle involves removing an electron from these metabolites and transferring it to the ETC, thereby reducing O 2 to O 2 [11]

In PIF3, a close homolog, the transactivation activity maps to a p53-like TAD featured by a xx motif (, hydrophobic residues) 57

T., Sokolowska, M