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Cells were lysed using RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% sodium dodecyl sulfate, 2 mM DTT, 2 mM sodium orthovanadate, 1 protease inhibitor cocktail [Roche])

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